Table 1 Removing the effect of stage removes Type I errors. Three replications of whole transcriptome samples from PB58 at 3 time points; 6 hpi, 26 hpi, and 38 hpi. A: In silico misalignment of the time points reveals the extent of expression differences that appear due to this misalignment (p < 0.01, FDR < 0.05). B: Application of cyclical regression covariates to correctly align the replications results in no differences in gene expression, the expected result of replication for a single strain in the haploid IDC stage