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Table 3 Plasmids used in this study

From: Genome-wide high-throughput signal peptide screening via plasmid pUC256E improves protease secretion in Lactiplantibacillus plantarum and Pediococcus acidilactici

Plasmids

Relevant characteristics

Source

pUC57

AmpR, pMB1 origin; cloning vector skeleton for shuttle vector

Bio Basic

pUC256

AmpR, pMB1 origin, p256 orgin

This study

pUC256E

AmpR, ErmR, pMB1 origin, p256 orgin, GusA reporter

This study

pUC256E-spLP_0373-NprB

pUC256E carrying NprB fused to spLP_0373 under Ppgm control

This study

pUC256E-spLP_0373-pro-NprB

pUC256E carrying pro-NprB fused to spLP_0373 under Ppgm control

This study

pUC256E-spLP_0373-PepJ

pUC256E carrying PepJ fused to spLP_0373 under Ppgm control

This study

pUC256E-spLP_0373-pro-PepJ

pUC256E carrying pro-PepJ fused to spLP_0373 under Ppgm control

This study

pUC256E-spLP_0373-PepA-Aa

pUC256E carrying PepA-Aa fused to spLP_0373 under Ppgm control

This study

pUC256E-spLP_0373-pro-PepA-Aa

pUC256E carrying pro-PepA-Aa fused to spLP_0373 under Ppgm control

This study

pUC256E-spLP_0373-PepA-An

pUC256E carrying PepA-An fused to spLP_0373 under Ppgm control

This study

pUC256E-spLP_0373-pro-PepA-An

pUC256E carrying pro-PepA-An fused to spLP_0373 under Ppgm control

This study

pUC256E-spLP_0373-PepG1

pUC256E carrying PepG1 fused to spLP_0373 under Ppgm control

This study

pUC256E-spLP_0373-pro-PepG1

pUC256E carrying pro-PepG1 fused to spLP_0373 under Ppgm control

This study

pUC256E-spLP_0600-PepG1

pUC256E carrying PepG1 fused to spLP_0600 under Ppgm control

This study

pUC256E-spsslipA-PepG1

pUC256E carrying PepG1 fused to spsslipA under Ppgm control

This study

pUC256E-spM6-PepG1

pUC256E carrying PepG1 fused to spM6 under Ppgm control

This study

pUC256E-spUsp45-PepG1

pUC256E carrying PepG1 fused to spUsp45 under Ppgm control

This study

pUC256E-spLIBRARY-PepG1

Plasmid library of pUC256E carrying PepG1 fused to homologous signal peptide under Ppgm control

This study