Fig. 1
From: Genion, an accurate tool to detect gene fusion from long transcriptomics reads
Genion pipeline. Preprocessing step produces alignment intervals for each input read. Paftools [30] is used to convert the mappings from SAM to PAF format. Mapped parts of the reads are masked and mapped again. Outputs of mapping steps are merged and converted to set of segments and annotated. Chimeric Read Identification step chains the annotated segments of each read and identifies the gene content. Chimeric Cluster Characterization step takes single-gene aligned chains to calculate gene expressions. Multi-gene aligned chains are clustered. Each cluster is statistically tested and FDR correction is applied on calculated p-values. For each cluster FiN and ff-igf scores are calculated. Clusters are characterized and ranked according to these scores and reported