Fig. 5

Cell line methylation status of CGI CpG79 located upstream of mir-486 as assessed by methylation specific PCR and direct bisulfite sequencing. The upper part is a schematic presentation of the CpG sites (vertical bars) amplified by the bisulfite sequencing primers. The transcription start site (+ 1) refers to the start of ANK1 (transcript variants 1–4). Arrows indicate location of MSP and qMSP primers, * represents the CpGs covered by the Infinium 450 k methylation array (cg08194989, cg26326633, cg00176210, respectively). For the lower part of the panel, black circles represent methylated CpGs (> 80% cytosine); grey circles represent partially methylated sites (20–80% cytosine) and white circles represent unmethylated sites (< 20% cytosine). The column to the right lists the methylation status of the respective cell lines as assessed by MSP analyses