Fig. 1
From: Genome-wide identification of MITE-derived microRNAs and their targets in bread wheat
A Scheme of the production site of MITE-derived miRNA 7 (highlighted in red) located at the intron 2 of TraesCS2B02G010300 gene. The DNA alignment sho ws the high sequence homology between the MITE-derived miRNA precursor, the most similar MITE identified (DTT_Hvul_ Pan_M801L24-1) and the mature MITE-derived miRNA 7. Highlighted in green is the Target Site Duplication (TSD) where the MITE was inserted and in yellow the mismatches between the intron 2 and MITE sequence. Also, mature miRNA and miRNA* as well as all small RNA reads mapped to the region as reported by Shortstack are shown below B Hairpin structure found in the production site of MITE-derived miRNA 7 in the intron 2 of the TraesCS2B02G010300 gene. C Scheme of the TraesCS2D02G449600.1 transcript showing a MITE-derived miRNA 7 (highlighted in red) target site at its 3’ UTR.The DNA alignment shows the high sequence homology between the target region, the most similar MITE (DTT_Taes_Pan_42j2-6) and the complementary position of the MITE-derived miRNA 7. Highlighted in green the TSD, in yellow the mismatches between the 3’ UTR, and the MITE and light blue the mismatches between the mature MITE-derived miRNA 7 and the complementary site in the 3’ UTR of TraesCS2D02G449600.1. D Signature plot of the cleavage site in the target transcript