Fig. 2

K-mer Analysis to study the guide composition. A Bar plot of the population of sgRNAs that contain a specific dinucleotide at position 8. B Box plot of target transcript expression values as a function of the nucleotide at position 8. C Barplot of negative log of univariate linear regression significance p-value for all monomers at all positions across the guide. D Bar plot of the feature contribution score for each feature in the Random Forest gini feature list.