Fig. 7

Common RT-PCR and qRT-PCR detection of selected miRNAs. A RT-PCR detection of miR49 (1–6 panuels), miR70 (8–13 panuels), miR1507a (14–19 panuels) and miR390d (21–26 panuels) in leaves of two lines inoculated with SC3 at 0 dpi,7 dpi and 14 dpi (The first three bands are from resistant line, the last three bands are from susceptible line in each group). It was cropped from the full-length gels which were presented in Additional file 9: Figure S6. The selected marker was 50 bp DNA ladder (The smallest two bands were 50 bp and 100 bp respectively) and the target band was about 65 bp. B-C For the qRT-PCR detection of the above 4 miRNAs in two lines, three biological replicates were set, the selected internal reference gene was U6, and 2^−ΔΔCT was used to calculate the expression level of miRNAs