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Fig. 5 | BMC Genomics

Fig. 5

From: Characterization of UDP-glycosyltransferase family members reveals how major flavonoid glycoside accumulates in the roots of Scutellaria baicalensis

Fig. 5

In vitro enzyme assays of SbUGTs and SbUGATs. A. HPLC analysis of SbUGTs using baicalein as a substrate in vitro enzyme assays. Top, oroxin A standard; EV, empty vector control; SbUGT1-3 and SbUGT7-9, assays with corresponding SbUGT proteins. B. MS and MS2 patterns of peak I products, which were identical to oroxin A standard. C. HPLC analysis of SbUGATs using baicalein as a substrate in vitro enzyme assays. Top, baicalin standard; EV, empty vector control; SbUGAT3-6, assays with corresponding SbUGAT proteins. D. MS and MS2 patterns of peak II products, which were identical to baicalin standard. E. The reaction catalyzed by SbUGT and SbUGAT using baicalein as a substrate. Red boxes indicated the different groups between sugar moieties

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BMC Genomics

ISSN: 1471-2164

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