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Table 1 Kinetic parameters and average FPKM values of roots and MeJA-induced roots of SbUGTs and SbUGATs

From: Characterization of UDP-glycosyltransferase family members reveals how major flavonoid glycoside accumulates in the roots of Scutellaria baicalensis

Enzymes

Km (μM)

Vmax (pkat mg protein− 1)

Vmax/Km

Average FPKM (Root)

Average FPKM (JA root)

SbUBGT

 SbUGT1

3.14

1027

327.07

12.53

28.18

SbUGT2

100.60

44503

442.38

245.11

417.07

 SbUGT3

2.67

3964

1484.64

23.87

34.87

 SbUGT7

–

–

–

8.73

6.93

 SbUGT8

–

–

–

50.98

100.63

 SbUGT9

–

–

–

53.69

86.67

SbUBGAT

 SbUGAT3

9.72

5607

576.85

2.38

2.63

SbUGAT4

10.20

6266

614.31

222.29

674.07 *

 SbUGAT5

15.44

8161

528.56

10.05

16.68

 SbUGAT6

12.32

7682

623.54

0.36

0.21

  1. One asterisk (*) indicates a significant difference (0.01 < P < 0.05) between the root and MeJA-induced root under t-test. The products of SbUGT7, SbUGT8 and SbUGT9 were very low or not detected at the linear reaction stage
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BMC Genomics

ISSN: 1471-2164

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