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Fig. 7 | BMC Genomics

Fig. 7

From: Characterization of pectin methylesterase gene family and its possible role in juice sac granulation in navel orange (Citrus sinensis Osbeck)

Fig. 7

Subcellular localization of CsPME proteins in tobacco leaves. a The green fluorescence of CsPME35:GFP, CsPME40:GFP, and CsPME52:GFP was overlapped with the red fluorescence of endoplasmic reticulum marker (HDEL:OFP), respectively. b The green fluorescence of CsPME6:GFP was co-localized with the red fluorescence of plasma membrane marker (CBL1n:OFP). c The green fluorescence of CsPME34:GFP was co-localized with the red fluorescence of vacuole membrane marker (CBL3n:OFP). d The red fluorescence of CsPME3:mCherry and CsPME21:mCherry was co-localized with the green fluorescence of RAmy3A:GFP, respectively. CsPME6, CsPME34, CsPME35, CsPME40, and CsPME52 were fused with GFP protein. CsPME3 and CsPME21 were fused with mCherry protein. ER-marker (HDEL:OFP), apoplast marker (RAmy3A:GFP), plasma membrane marker (CBL1n:OFP), and vacuole membrane marker (CBL3n:OFP) were utilized for co-localization analysis and imaging

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BMC Genomics

ISSN: 1471-2164

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