Fig. 8
Transcription factor CsRVE1 bound to the promoter of CsPME3 and activated its activity. a Yeast one-hybrid assay suggested the interaction between CsRVE1 and the promoter of CsPME3. b Dual luciferase transcriptional activity assay revealed the activation of proCsPME3 by CsRVE1. c Subcellular localization of CsRVE1 in tobacco leaves. CsRVE1 was fused with GFP, and nucleolus marker (FIB2:mCherry) was utilized for co-localization analysis and imaging. d. Spatial and temporal expression profiles of CsRVE1 in navel orange fruits. The color scale of heatmap represents the log2-transformed FPKM values. e. Expression patterns of CsRVE1 under low temperature stress. CsEF1 was used as internal control, and three biological replicates were performed. Three individual biological replicates were performed for Dual luciferase transcriptional activity assay and qRT-PCR, data were shown as means ± SE of three biological replicates, and bars with different lowercase letters indicated significant differences (p < 0.05 of one-way ANOVA in SPSS software)