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Table 1 Alignment statistics of the 12 milk somatic cells samples collected from 6 Holstein dairy cowsa

From: Identification of novel alternative splicing associated with mastitis disease in Holstein dairy cows using large gap read mapping

Group

Sample ID

Total reads mapped

Uniquely mapped reads%

Non - specifically mapped reads%

Unmapped reads%

Healthy

50Ab

22,791,224

91.35

5.19

3.46

50Cc

12,757,084

81.84

7.50

10.67

50Ed

11,257,761

85.75

5.56

8.70

50Ge

9,041,681

78.70

4.87

16.43

70Af

11,737,755

87.18

12.34

0.47

70Eg

16,124,047

95.17

4.36

0.48

 

Total = 83,709,552

Av = 86.66

Av = 6.63

Av = 6.70

Mastitic

50Bb

18,884,191

93.19

5.20

1.61

50Dc

26,510,003

92.70

6.47

0.83

50Fd

14,613,683

91.21

5.67

3.11

50He

15,174,409

90.40

6.86

2.75

70Cf

10,692,707

95.33

3.93

0.74

70Gg

12,365,761

95.63

3.95

0.41

 

Total = 98,240,754

Av = 93.08

Av = 5.35

Av = 1.58

  1. aSamples were aligned to the ARS_UCD1.2 bovine reference genome; bThese samples were collected from the same cow (3rd lactation, 74 DIM); cThese samples were collected from the same cow (2nd lactation, 44 DIM); dThese samples were collected from the same cow (3rd lactation, 178 DIM); eThese samples were collected from the same cow (2nd lactation, 133 DIM); fThese samples were collected from the same cow (2nd lactation, 7 DIM); gThese samples were collected from the same cow (1st lactation, 236 DIM)