Fig. 1
From: In vitro and in silico parameters for precise cgMLST typing of Listeria monocytogenes
Experimental plan (A) and data analyses (B) aiming at controlling a dataset of downsampled paired-end reads (i: n = 420 paired-end reads), as well as identifying statistically (ii) and confirming significant parameters (iii) explaining the Listeria monocytogenes cgMLST precision (identical alleles against circular reference genomes: n = 2520 cgMLST typing) among in vitro (i.e. tested reference genomes, successive platings, as well as replicates of DNA extraction and sequencing) and in silico (i.e. targeted depth of coverage, read depth and breadth of coverage, assembly metrics, cgMLST workflows, identified alleles against schema) parameters (n = 57 parameters of interest)