Fig. 2

Analysis of Chromosome – wise distributed MARs. The MAR DNA from posterior silk glands extracted from day 1, day 5 and day 7 were sequenced using Illumina platform and mapped against the Bombyx mori annotated genome downloaded from Silkbase. A The depth (left) and coverage (right) of the mapped sequenced reads was analysed using PlotCoverage. B Chromosome wise distribution of MARs is shown. C Lengths of the MARs in the three datasets were derived from the enriched MAR peaks data. D Scatter plot of the density of MARs plotted against the gene densities for each chromosome is shown. E The enrichment of MAR regions on each chromosome was obtained using MACS2 callpeak. Chromatin loop length for each chromosome was obtained by calculating the distance between the MAR regions and the average loop length in each dataset is depicted in the graph. F Common and unique MARs among the three datasets are shown. SG1 and SG5 consist of less number of MARs. They are rich in repetitive regions. The number of MARs and unique MARs are more in SG7