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Table 7 Annotation of the CDSs in pCP-OS1

From: Analysis of the complete genome sequences of Clostridium perfringens strains harbouring the binary enterotoxin BEC gene and comparative genomics of pCP13-like family plasmids

No. of CDS

Position (nt)

1–54,536

Size (bp)

(strand)

Identical ORFs of pCP13

Gene name

Annotation/putative function

1

1–753

753(+)

PCP01

parA

Type I partitioning system ATPase

2

812–2092

1281(+)

PCP02

parB

Type I partitioning system centromere binding protein

3

2208–2570

363(+)

PCP03

–

Hypothetical

4

2730–3308

579(−)

PCP12

–

Hypothetical with SMC_N superfamily domain

5*

3620–4033

414(+)

–

–

Hypothetical

6*

4091–5125

1035(+)

–

–

ABC transporter permease

7*

5151–6326

1176(+)

–

–

ABC transporter permease (FtsX)

8*

6323–7006

684(+)

–

–

ABC transporter ATP-binding protein

9

7270–7842

573(+)

PCP15

resP

Serine recombinase/resolvase (ResP)

10*

8213–8971

759(+)

–

–

Hypothetical

11*

8986–9861

876(+)

–

–

Hypothetical

12

9926–10,570

645(−)

PCP18

–

Hypothetical

13

10,582–10,896

315(−)

PCP19

–

PadR family transcriptional regulator

14

11,456–11,659

204(+)

PCP24

–

Hypothetical

15

11,733–12,122

390(+)

PCP25

–

Hypothetical

16*

12,618–12,737

120(−)

–

–

Hypothetical

17*

13,223–15,622

2400(−)

–

becB

Binary enterotoxin component b

18*

15,641–16,900

1260(−)

–

becA

Binary enterotoxin component a

19*

17,193–17,786

594(+)

–

–

Sigma-70 family RNA polymerase sigma factor

20*

17,779–17,913

135(+)

–

–

Hypothetical

21*

18,188–18,376

189(+)

–

–

Hypothetical

22*

18,381–18,599

219(+)

–

–

Hypothetical

23*

18,777–18,884

108(−)

–

–

Hypothetical

24*

19,292–19,894

603(+)

–

–

Recombinase family protein

25*

20,361–20,663

303(+)

–

–

Hypothetical

26

20,920–21,417

498(−)

PCP34

pcpT

Hypothetical

27

21,473–24,283

2811(−)

PCP35/36

pcpS/R

PcpS: Hypothetical, PcpR: ImmA/IrrE family metallo-endopeptidase

28*

24,416–25,345

930(−)

–

–

Restriction enzyme

29*

25,365–26,855

1491(−)

–

–

Eco571 restriction-modification methylase domain-containing protein

30

27,021–27,239

219(−)

PCP38

pcpP

Hypothetical

31

27,257–28,390

1134(−)

PCP39

pcpD2

Putative relaxase

32

28,393–28,800

408(−)

PCP40

pcpO

N-terminal CopG-like ribbon-helix-helix protein (35% coverage of aa sequence)

33

28,804–29,073

270(−)

PCP41

pcpN

Hypothetical

34

29,402–30,265

864(−)

PCP43

pcpL

Hypothetical

35

30,443–31,558

1116(−)

PCP44

pcpB1

Putative peptidoglycan hydrolase

36

31,691–32,383

693(−)

PCP45

pcpK

Hypothetical C-terminal Ntf-like transpeptidase domain protein (6% coverage of aa sequence)

37

32,388–34,286

1899(−)

PCP46

pcpB4

VirB4-like ATPase

38

34,304–36,415

2112(−)

PCP47

topA

Topoisomerase III

39

36,460–37,104

645(−)

PCP48

pcpJ

Hypothetical

40

37,220–37,501

282(−)

PCP49

pcpI

Hypothetical

41

37,504–39,633

2130(−)

PCP50

pcpB6

VirB6-like

42

39,630–42,371

2742(−)

PCP51

pcpD4

VirD4-like coupling protein

43

42,358–42,570

213(−)

PCP52

pcpH

Hypothetical

44

42,637–42,873

237(−)

PCP53

pcpG

Hypothetical

45

42,937–43,131

195(−)

PCP54

pcpF

Hypothetical

46

43,124–43,576

453(−)

PCP55

pcpE

Spo0A-homologue

47

43,695–44,291

597(−)

PCP56

pcpD

Sortase

48

44,535–48,641

4107(−)

PCP57

cnaB

Collagen adhesion protein

49

48,786–49,136

351(−)

PCP58

pcpC

PemK toxin (type II toxin-antitoxin system)

50

49,260–50,327

1068(−)

PCP59

pcpB

Hypothetical

51

50,373–50,858

486(−)

PCP60

pcpA

Helix-turn-helix containing DNA-binding regulatory protein

52

51,171–52,274

1104(+)

PCP61

regC

LexA-like transcriptional regulator, similar to RegC from pCW3

53

52,388–52,834

447(−)

PCP62

–

Hypothetical

54

52,863–53,780

918(−)

PCP63

rep

replication protein

  1. The CDS of pcpM was not annotated (identified) by Prokka and was excluded from this table. Asterisks indicate unique CDSs in pCP-OS1 compared to pCP13