Transcriptome analysis of blastoderms exposed to prolonged egg storage and short periods of incubation during egg storage

Table 3 Summary of Ingenuity Pathway Analysis results for NSR vs. CR comparison

Network Score	Top Functions
42	Cell Cycle, Cellular Assembly and Organization, DNA Replication, Recombination, and Repair
42	Protein Synthesis
42	Cell Cycle
Regulators in Network		Score	Targets	Functions
RABL6		13	12	Cell cycle progression, Embryo death
CDKN1B, E2F2, LIN9, MYBL2, RABL6, RBL1, TASP1		12	37	G1/S phase transition
E2F2, LIN9, mir-145		10.4	24	Cell cycle progression, Organismal death
Upstream Regulator	Fold	Molecule Type		Activation z-score	P Value
TWNK	−1.811	enzyme		−3	5.17E-06
ACTL6A	−1.699	other		−2.236	0.00805
GNL3	−1.713	other		−2.236	0.0148
TCF3	1.645	transcription regulator		2.613	8.18E-06
Master Regulator	Fold	Molecule Type		Activation z-score	P Value
BIRC5	−1.985	other		−8.472	0.0001
SRSF2	−2.236	transcription regulator		−6.146	0.0001
PDCD4	2.187	other		−5.543	0.0001
TFDP1	−1.871	transcription regulator		−5.489	0.0001
CCNE1	−2.423	transcription regulator		−5.384	0.0001
COPS2	−1.639	other		5.514	0.0001
APLP2	1.835	other		5.846	0.0001
HEXIM1	−1.604	transcription regulator		5.996	0.0001
UCHL1	−1.581	peptidase		6.068	0.0001
BARD1	1.961	transcription regulator		6.072	0.0001

The top three networks (network score > 40) and top three regulator effect networks (consistency score > 0, target number > 3, |z-score| > 2, p-value < 0.05) are shown. The top five activated (z-score > 2) and inhibited (z-score < −2) upstream regulators and master regulators in causal networks are shown (|fold change| > 1.5, |z-score| > 2, p-value < 0.05). Only upstream regulators and master regulators exhibiting differential expression between NSR and CR groups are displayed

ISSN: 1471-2164