Fig. 2

Consistency of IPDs between WGA and native samples for short motifs. A-C Concordance between the IPDs in the WGA (colored red) and native (blue) samples (replicate 1 in the 1st row, replicate 2 in the 2nd row). The charts show the IPD distributions represented by an error bar plot in the motifs and their surrounding 10 nucleotides in the x-axis. Nearly identical IPD distributions are obtained from the two biological replicates (0.99 < Pearson’s correlation coefficient; Supplementary Fig. 2C). Figure A displays GATC, where N6-methyladenine is prevalent in E. coli. Figure B and C show motifs that were reported to have N6-methyladenine in C. elegans. D IPD distributions in the two WGA replicates around the four 2-mer motifs (GA, AT, AG, and AA) that have adenines and occur in the 4-mer motifs, GATC, GAGG, and AGAA in Figure A-C. The A’s average IPD in GA are higher than those of the other three 2-mer motifs, and almost concord with the average IPDs of A in GATC and AGAA, but is much smaller than the A’s average IPD in GAGG