Fig. 4

Overexpression of HLA-C in colorectal cancer cells (SW480). A Map of HLA-C overexpression plasmid, pcDNA-HLAC. B Relative mRNA expression levels of HLA-C measured by qRT-PCR in HLA-C overexpressing stable cells (Over-HLA) and SW480 cells. qRT-PCR-measured and ACTB normalized mRNA expression at the cell level was compared between Over-HLA and SW480 cells. C Relative protein levels of HLA-C determined by western blot in Over-HLA and SW480 cells. The blots for HLA-C and β-actin were obtained from duplicated gels using 30 µg of proteins prepared from each Over-HLA and SW480 sample. The full-length blots are presented in a Fig. S3. D The viability of Over-HLA and SW480 cells. Absorbance at 450 nm was measured at 24, 48, and 72 h after incubation. Notes: Group differences were assessed by the Wilcoxon rank-sum test. *P < 0.05, **P < 0.01, ***P < 0.001 vs control