Fig. 1

Transcriptomic and epigenomic changes associated with onset of salmon maturation (A), Induction of maturation through photoperiod manipulation and sampling time points. Animals were managed via photoperiod to synchronise the timing of commitment into maturation. 4 fish were sampled at each of the T1 (before long day photoperiod signal) and T2-T4 time points (during maturation) in 2 weeks intervals to control for variation between individuals. Gonadosomatic index (GSI) increased gradually from T2 till the last sampling event at T4 indicating active response towards maturation in these animals (B), Tissue collection and multi-omics analyses. Samples from the pituitary gland, ovary and liver were collected at each sampling event. Transcriptome and DNA methylome data in the three tissues along with accessible chromatin data in liver were collected (C), Significant transcriptome remodelling driving salmon maturation. 9 MA plots (showing differentially expressed genes (DEGs) in pituitary, ovary and liver (FDR < 0.05 and log₂fold change > ±1) at T2, T3 and T4 during maturation compared to control samples at T1