Fig. 3

Multi-tissue DNA methylome maps reveal differentially methylated genes with key roles in salmon maturation (A), Changes in CpG methylation across four genomic regions per tissue, promoters were defined as regions located 5 kb upstream of transcription start sites (TSSs) (B), Significantly enriched gene ontology (GO) terms among ovary hypermethylated genes (n = 1156) against background of ovary genes (hypergeometric test, Bonferroni-adjusted P < 0.05) (C), Significant overlap between ovary DMGs and DEGs (D), Methylation and expression directionality at gene bodies confirms that gene body methylation is associated with gene activation (E), Significantly enriched GO terms (adjusted P < 0.05) among the list of 148 hypermethylated genes in ovary (F), Heatmaps showing significant increases in CpG methylation and expression of genes driving GO enrichment shown in part F. G Heatmaps showing significant increases in CpG methylation and expression of transcription factors and maturation candidate genes and among the list of the 148 genes. H CpG methylation profiles for the GRM8 gene. The methylation plot shows percentages of methylated CpGs along with coverage depth at each CpG site. Pink rectangles represent the DMR and genomic coordinates are indicated below the density plot