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Fig. 3 | BMC Genomics

Fig. 3

From: Weighted gene co-expression network indicates that the DYNLL2 is an important regulator of chicken breast muscle development and is regulated by miR-148a-3p

Fig. 3

The effect of DYNLL2 and miR-148a-3p on CPMs differentiation. A Relative expression of DYNLL2 after overexpression in CPMs. B The changes of differentiation marker genes MYOD, MYOG, and MYHC after DYNLL2 transfection in CPMs. C MYHC staining after transfection of DYNLL2. D Changes in the differentiation marker MYHC protein after DYNLL2 transfection of CPMs (The order of the samples, lanes 1-2: pcDNA3.1, DYNLL2). E The relative expression of miR-148a-3p after transfection with miR-148a-3p mimics. F The changes of differentiation marker genes MYOD, MYOG, and MYHC after miR-148a-3p transfection in CPMs. G MYHC staining after transfection with the miR-148a-3p mimic in CPMs. H Changes in the differentiation marker MYHC protein after transfection with the miR-148a-3p mimics (The order of the samples, lanes 1-2: mimic NC, miR-148a-3p mimic). I Changes in the differentiation marker MYHC protein after co-transfection of miR-148a-3p mimics and DYNLL2. (The order of the samples, lanes 1-3: mimic NC, miR-148a-3p mimic, miR-148a-3p mimic+DYNLL2). The data are expressed as the mean ± S.E.M. (* p <0.05; ** p <0.01, *** p <0.001)

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BMC Genomics

ISSN: 1471-2164

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