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Fig. 5 | BMC Genomics

Fig. 5

From: Integrative analysis of circRNA, miRNA, and mRNA profiles to reveal ceRNA regulation in chicken muscle development from the embryonic to post-hatching periods

Fig. 5

Experimental validation of circular RNAs. A Divergent primers amplify circRNAs in cDNA, but not genomic DNA (gDNA). Black triangles represent convergent primers and white triangles represent divergent primers. The gels were cropped to improve the clarity and conciseness of the presentation (B) Sanger sequencing confirmed the back-splicing junction sequence of circRNAs. C qRT-PCR showing resistance of circRNAs to RNaseR digestion. (D) The qRT-PCR validation of six differentially expressed circRNAs in six development time points

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BMC Genomics

ISSN: 1471-2164

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