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Table 1 Comparison of sequencing Run116 on both the MiSeq and the GridION

From: Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeq

 

Run116

 

MiSeq

GridION

Runtime (hrs.)

36

21

No. of samples sequenced

93

93

Data obtained

7246.3 MB

1999,4 MB

Consensus genomes

93

93

Average coverage (X)

94.34%

72.96%

Passing GISAID QC (> + 80%)

83 (89.2%)

29 (27.9%)

Clusters passing QC

70%

–

Q30 score

73.1%

–

Pores on flowcell

–

1012 pores

  1. The table above summarizes the sequencing of Run116 on both the MiSeq and the GridION. The sequencing runtime for the MiSeq was 36 h, whilst that of the GridION was 21 h. The MiSeq had a Q30 score of 73.1% with 70% of the clusters passeing QC The flowcell used for the GridION had 1012 pores available for sequencing. Of the 93 samples sequenced by both platforms, 93 consensus genomes were produced by each. Consensus genomes from the MiSeq had an average coverage of 94.34% with 89.2% having a coverage of 80% and over. Consensus genomes from the GridION had an average coverage of 72.96% with 27.9% having a coverage of 80% and over
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BMC Genomics

ISSN: 1471-2164

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