Anaplasma phagocytophilum in Marmota himalayana

BMC Genomics

Table 3 Universal primers for the 16S rRNA gene, and specific primers for the 16S rRNA and groESL genes of A. phagocytophilum, and primers for Y. pestis genes

Target gene	PCR stage	Primer name	Sequence	Product length	Reference
16S rRNA	-^a	27F	AGA GTT TGA TCM TGG CTC AG	varied	[28]
16S rRNA	-^a	1492R	TAC GGY TAC CTT GTT ACG ACT T	varied	[28]
16S rRNA	Primary PCR	Eh-out1	TTG AGA GTT TGA TCC TGG CTC AGA ACG	653	[31, 32]
	Primary PCR	Eh-out2	CAC CTC TAC ACT AGG AAT TCC GCT ATC	653
	Nested PCR	Eh-gs1	GTA ATA CT GTA TAA TCC CTG	282
		Eh-gs2	GTA CCG TCA TTA TCT TCC CTA	282
		HGA1	GTC GAA CGG ATT ATT CTT TAT AGC TTG	389
		HGA2	TAT AGG TAC CGT CAT TAT CTT CCC TAC	389
groESL	Primary PCR	HS1	TGG GCT GGT A(A/C) TGA AAT	1431	[33]
	Primary PCR	HS6	CCI CCI GGI ACI A(C/T) ACC TTC	1431
	Nested PCR	HS43	AT(A/T) GC(A/T) AA(G/A) GAA GCA TAG TC	480
	Nested PCR	HS45	ACT TCA CG(C/T) (C/T) TCA TAG AC	480
caf1	- ^a	fra-1F	GGAACCACTAGCACATCTGTT	249	[27]
caf1	- ^a	fra-1R	ACCTGCTGCAAGTTTACCGCC	249
pla	- ^a	pla-2F	ACTACGACTGGATGAATGAAAATC	456
pla	- ^a	pla-2R	GTGACATAATATCCAGCGTTAATT	456

ISSN: 1471-2164