Target gene | PCR stage | Primer name | Sequence | Product length | Reference |
---|---|---|---|---|---|
16S rRNA | -a | 27F | AGA GTT TGA TCM TGG CTC AG | varied | [28] |
1492R | TAC GGY TAC CTT GTT ACG ACT T | ||||
16S rRNA | Primary PCR | Eh-out1 | TTG AGA GTT TGA TCC TGG CTC AGA ACG | 653 | |
Eh-out2 | CAC CTC TAC ACT AGG AAT TCC GCT ATC | ||||
Nested PCR | Eh-gs1 | GTA ATA CT GTA TAA TCC CTG | 282 | ||
Eh-gs2 | GTA CCG TCA TTA TCT TCC CTA | ||||
HGA1 | GTC GAA CGG ATT ATT CTT TAT AGC TTG | 389 | |||
HGA2 | TAT AGG TAC CGT CAT TAT CTT CCC TAC | ||||
groESL | Primary PCR | HS1 | TGG GCT GGT A(A/C) TGA AAT | 1431 | [33] |
HS6 | CCI CCI GGI ACI A(C/T) ACC TTC | ||||
Nested PCR | HS43 | AT(A/T) GC(A/T) AA(G/A) GAA GCA TAG TC | 480 | ||
HS45 | ACT TCA CG(C/T) (C/T) TCA TAG AC | ||||
caf1 | - a | fra-1F | GGAACCACTAGCACATCTGTT | 249 | [27] |
fra-1R | ACCTGCTGCAAGTTTACCGCC | ||||
pla | - a | pla-2F | ACTACGACTGGATGAATGAAAATC | 456 | |
pla-2R | GTGACATAATATCCAGCGTTAATT |