Fig. 3

Female (A) and male (B) interaction networks built from eQTLs and known genetic and physical associations. The central networks in each panel represent the sex-separated, filtered interaction networks generated by incorporating eQTL associations calculated from expression differences, between ethanol and control conditions, of genes with a statistically significant line-by-treatment (LxT) term in the ANOVA model, to known genetic and protein–protein or RNA–protein physical interactions from the FlyBase interaction database. Pink nodes represent the genes from the LxT set. Yellow nodes represent genes either containing or within 1,000 bp of the eQTL variant. Cyan nodes represent genes with known genetic or physical interactions to the rest of the network. Blue edges represent the eQTL associations from this study. Green and orange edges represent known genetic and physical associations from the Flybase interaction database. Individual inlets of genes around the central network are MCODE-generated modules of genes. Annotations of the inlets are based on statistically enriched pathways for genes within these modules. Terms with Benjamini–Hochberg FDR adjusted P-value < 0.05 in the statistical overrepresentation test were considered statistically significant