Fig. 1From: A random priming amplification method for whole genome sequencing of SARS-CoV-2 virusSchema of random amplification methods for whole genome assembly of SARS-Cov-2 virus genome. Method 1 (H-P) is based on the RT-PCR step with random hexamers primer (6Ns) followed by phi29 polymerase isothermal amplification in the presence of 6Ns primer and then library preparation for Illumina sequencing. Method 2 (K-P), random octamer tagged with 20 nucleotide known tag sequence (5`-GACCATCTAGCGACCTCCACNNNNNNNN-3`) (K-8Â N) was used for RT-PCR step, followed by phi29 polymerase isothermal amplification in the presence of tagged primer K-8Â N and then library preparation and Illumina sequencing. Method 3, Sequence-Independent, Single-Primer Amplification (SISPA) technique, followed by library preparation and Illumina sequencing. Method 4 (S-P), following SISPA amplification (Method 3), phi29 polymerase isothermal amplification in the presence of random hexamers (6Ns) was applied and then used for Illumina sequencingBack to article page