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Fig. 2 | BMC Genomics

Fig. 2

From: Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages

Fig. 2

pgRNA CRISPR library for lncRNA and pc-genes. A (Upper panel) Diagram of the CD19 gene indicating the target sequence of CD19 pgRNAs (sgRNA1 and sgRNA2, from left to right). (Lower panel) Flow cytometry analysis of fluorescence intensity of the CD19 protein in BLaER1-Cas9 cells infected with sgRNAs and pgRNAs. The relative Stain Index of the different infected cells compared to the maximum expression level of CD19 in control cells (BLaER1-Cas9 cells infected with pDECKO-GFP [11]) is represented. CD19 expression is reduced between 30 and 95% upon infection of sgRNAs. The infection of pgRNAs induces a consistent reduction of CD19 signal up to 95% with all pgRNAs tested. B Schematic diagram showing the position of pgRNAs targeting lncRNAs (targeting the promoter and the transcription start site) and pc-genes (targeting coding exons). C CRISPR library composition (number of targets of each biotype and pgRNA pairs designed per target)

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BMC Genomics

ISSN: 1471-2164

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