The Fasciola hepatica miRnome displays temporal expression during the life cycle within the mammalian host. (a) Heatmap of the expression of the miRnome across triplicate RNA samples of newly excysted juveniles (NEJ), immature fluke (JUV) and adult parasites (AD) where minimum expression (low expression – blue) and maximum expression (high expression – red) of the individual miRNAs is represented. Hierarchical clustering of the average values of miRNAs and sample types was performed using One Pearson correlation. (b) Parallel coordinates of F. hepatica miRNA expression (logCPM) separately analysed based on category; miRBase miRNAs (blue), other published non-miRBase miRNAs (red) and novel miRNAs determined in this study (yellow). (c) Principal components analysis (PCA) plots of the total miRNA expression across all sample types and miRNAs in 3D (From left to right – front view, side view, top view). Size of the nodes depicts total miRNA input (miRNA presence) between sample types NEJ (green scale), immature flukes (orange scale) and adult parasites (blue scale).