Fig. 3

Expression profiles of lncRNAs, miRNAs and mRNAs in 14-day osteogenic differentiation of rBMMSCs. A, Detection of differentially expressed lncRNAs, miRNAs and mRNAs. B, Three volcano maps of differentially expressed lncRNAs, miRNAs and mRNAs with the standards of P < 0.05 and |Fold change|>2. Filtered transcripts were in gray, transcripts with non-significant differences were in blue, and transcripts with significant differences were in red. log₂ Fold change was displayed on the horizontal axis, and -log₁₀ (P value) was displayed on the vertical axis. C, lncRNAs expression profile in heat map. D, miRNAs expression profile in heat map. E, mRNAs expression profile in heat map. These three heat maps were constructed based on expression values of all expressed lncRNAs, miRNAs and mRNAs detected by RNA-seq, respectively. The expression values from − 2 to 2 were depicted in line with the color scale representing the intensity increased from green to red. Three osteogenic induced groups and three control groups were included, each column represented one sample and each row indicated one transcript. Bioinformatics software (http://www.bioinformatics.com.cn/) was used to create the maps in Fig. 3B ~ E