Fig. 3

iTRAQ–LC–MS/MS data validation by Western blotting (WB). a Abundance of three upregulated [IGF-1 (full-length blots/gels are presented in Additional file 5), RCAN1 (full-length blots/gels are presented in Additional file 6), and DHCR24 (full-length blots/gels are presented in Additional file 7)] and two downregulated [CDK1 (full-length blots/gels are presented in Additional file 8) and STMN1 (full-length blots/gels are presented in Additional file 9)] proteins were analyzed by WB. β-Tubulin (full-length blots/gels are presented in Additional file 8) served as the internal reference. (Some positions of marker were in the middle and some were on the edge of the gels. To display the images more aesthetically, we have cropped them.) b Quantitative results for proteins. Values represent mean ± SEM (n = 3/group). Statistical significance was assessed using Student’s t-test; P < 0.05