Fig. 1

Simulation of a bulk segregant analysis with different recombination rates. The frequency of the alternate allele for each marker position is shown in two contrasted pools of segregant lines displaying high phenotype (A) and low phenotype (B). The difference in allele frequency among the pools and the smoothed statistics for the window corresponding to 3 Mbp (line) led to the detection of a QTL simulated in the middle of the 100 Mbp model chromosome (C) in different species with different recombination ratios (λ): pearl millet (λ = 0.90), rice (λ = 1.30) and foxtail millet (λ = 2.15). The results correspond to simulations using binomial distribution in the simulation of sequencing noise and QTL effect equivalent to 20% of the phenotypic variance (k = 1). The first graph in A, B and C shows the results in absence of a QTL effect