Fig. 4

Multiple sequence alignment of repeats identified in the 4 precursor proteins and an extended region of G11-514P containing the single linusorb domain with some flanking residues. Repeats are numbered on the left of the alignment and the name of linusorb domain in each repeat is shown on the right, while repeats containing undetected linusorb-like domains (LLDs) are marked by “?”. Linusorb domain sequences are italicized in the alignment, except LOs E1 – E3, the 3 glycine-containing analogues of LOs B1 – B3, which are underlined. Consensus sites flanking the linusorb(−like) domains are highlighted in bold. The scale on the top marks the starting position (0) of the linusorb domain, and the flanking sites are numbered as minus towards the N-terminus and as plus towards the C-terminus. Colors of amino acids employed the hydropathicity color scheme in which hydrophobic amino acids (YVMCLFIW) are colored black, hydrophilic (RKDENQ) are blue and neutral (SGHTAP) are green