Fig. 2

Transcriptional activity detection and subcellular localization of GsMYB7 protein. A Transcriptional activation of GsMYB7 protein. B Subcellular localization of GsMYB7 protein. The ORF of GsMYB7 gene was fused with the GAL4 DNA binding region of pGBKT7 vector and expressed in yeast strain Y₂H Gold. After the transformation, the two positive clones were transferred to SD/-TRP solid medium, and the other group was added with substrate X-a-Gal. The constructed fusion expression vector of pCAMBIA1302-eGFP-GsMYB7 and empty vector pCAMBIA1302-eGFP were transformed into Agrobacterium tumefaciens GV3101, respectively, cultured with the virus protein P19 in equal volume, and then injected into 4-week old Nicotiana tabacum L epidermal cells. After cultured for 48 h later, the leaves were observed by laser confocal microscopy (Carl Zeiss, Jena, Germany)