Fig. 4

Detection of transgenic soybean lines. A PCR identification using Bar gene primers (Additional file 3: Table S10). B The gene expression of transgenic soybean lines was detected by qRT-PCR. (C) Tolerance of transgenic plants to herbicides. The blade side with the tick is the side to which the herbicide is applied, and the left side is untreated with herbicide. The relative gene expression levels of HC6 (WT) and GsMYB7 transgenic soybean lines were calculated by 2^{^−△△CT} method in three separate biological experiments. WT: wild type, HC6; L1, L2, L3, L4, L5: GsMYB7 transgenic T₃ generation soybean