Fig. 9
Expression patterns of downstream genes regulated by GsMYB7. Soybean plants were cultured in CaCl2 solution supplemented with 15 μM AlCl3 (pH4.5) for 8 h. The expression patterns of downstream responsive genes regulated by GsMYB7 were detected. Using soybean Actin3 as the internal reference gene, qRT-PCR was used to detect the gene expression level before and after aluminum treatment. The asterisks indicated that the transcripts of investigated genes in GsMYB7 transgenic lines were significantly different from those of wild type treated with and without AlCl3 (*P < 0.05; **P < 0.01). All the information of gene sequences were from the NCBI database (https://www.ncbi.nlm.nih.gov/) shown in Additional file 1: Table S5