Fig. 5

Interactions between B. fragilis and MGEs, as well as, the distribution of protospacers in NC_011222. MGEs containing protospacers are shown in ovals with plasmids highlighted in red and phages in orange. (A) The spacer-MGE network includes spacers identified from Zhao2019 and the genomes of their MGE targets (if protospacer were identified in any known MGE reference genomes). Spacers identified in different types of CRISPR-Cas systems are shown in rectangles of different colors (Type I-B in light green, Type II-C in dark green, and Type III-B in blue). (B) The host-MGE network includes spacer-contributing B. fragilis isolates from Zhao2019 only. Hosts are shown in hexagons with B. fragilis isolates from different individuals shown in different shades of green; four phage MGEs are highlighted with labels: P1 (uvig_425872, Siphoriridae), P2 (uvig_422350, Siphoviridae), P3 (k141_68_round8-12 1718861, Microviridae), and P4 (NC_011222, Siphoriridae). (C) The distribution of protospacers in NC_011222, with green and red vertical lines representing protospacers matching spacers found in Type I-B and Type II-C CRISPR-Cas systems, respectively (gray arrows represent the genes). (D) PAMs detected in the downstream regions of the protospacers associated with Type I-B and Type II-C CRISPR-Cas systems, respectively