Fig. 1

Evidence for lineage-specific immune cell differential methylation. A Principal component analysis plot of porcine immune cells based on cDMR methylation rate. B Methylation rates across the CD3 gene locus for T cell populations (solid lines) and other cell types (dashed lines). Gray boxes indicate cDMRs, and black dots indicate CpG coordinates. C Methylation rates surrounding the CD8A transcription start site for expressing and non-expressing cell types. Letters in legend indicate significant differences in cDMR methylation (gray boxes) between cell types. D Heatmap of immune cell lowly methylated region (cLMR) enrichment scores in genomic features. Numbers indicate enrichment p-values. cDMR = cell differentially methylated region