Fig. 5

Characterization of the predicted circRNAs. A Amplification of the circRNAs using divergent primers with cDNA. Lanes 1, 3 and 4 refer to the cDNA samples transcribed by random hexamer primers, and lane 2 refers to the cDNA samples transcribed by oligo (dT)₁₈. The cDNA used in lane 3 was synthesized by total RNA digested with RNase R, and lane 4 was a control. : represents back-splicing sites. B Verification of circRNA expression patterns. The circRNA expression level of RNA-seq was calculated as Log₅₀(TPM), and the RT–qPCR data were computed as the mean ± S.E.M. TPM: transcripts per million