Fig. 2

Study design. Here we aimed to investigate and reduce the influence of gDNA contamination on gene expression. Total RNA and gDNA were extracted from a human HapMap lymphoblast cell line, and total RNA was divided into two groups: one treated with DNase and the other not treated with DNase. The gDNA was added to the DNase-treated RNA to achieve concentrations of 0% to 10%. These RNA/DNA mixtures and the non-DNase-treated RNA were prepared to construct the RNA-seq libraries using the Ribo-Zero and Poly (A) Selection methods. Each treatment was performed in triplicate, and 36 libraries were prepared. Sequencing data (50-bp reads) were generated using an Illumina HiSeq2000