Table 1 Summary of piRNA and mRNA transcriptome data of Takifugu flavidus gonads
Sample group | FCG | FTG | MCG | MTG | |
|---|---|---|---|---|---|
piRNA-seq | clean bases | 0.983G | 0.668G | 1.032G | 0.841G |
total reads | 17,695,205 | 11,741,016 | 17,391,118 | 13,693,446 | |
uniq reads | 2,915,273 | 2,660,367 | 2,515,290 | 3,116,492 | |
error rate (%) | 0.02 | 0.02 | 0.02 | 0.02 | |
Q20 (%) | 99.62 | 99.64 | 99.55 | 99.59 | |
Q30 (%) | 98.86 | 98.94 | 98.75 | 98.79 | |
GC content (%) | 48.9200 | 49.3300 | 48.8600 | 49.1900 | |
uniq- piRNAs | 507,004 | 472,764 | 335,106 | 530,323 | |
numbers of piRNA (reads ≥ 500) | 723 | 365 | 403 | 345 | |
piRNA clusters | 5396 | 5385 | 5297 | 5418 | |
mRNA-seq | raw reads | 20,935,814 | 23,188,308 | 23,438,065 | 20,223,260 |
clean reads | 20,420,252 | 22,290,909 | 22,827,658 | 19,650,444 | |
total mapped | 34,740,218 (85.06%) | 38,356,040 (86.04%) | 39,307,194 (86.10%) | 33,651,476 (85.63%) | |
clean bases | 6.13G | 6.69G | 6.85G | 5.90G | |
error rate (%) | 0.02 | 0.02 | 0.02 | 0.02 | |
Q20 (%) | 98.28 | 98.2 | 98.04 | 98.09 | |
Q30 (%) | 94.91 | 94.91 | 94.32 | 94.62 | |
GC content (%) | 52.31 | 53.58 | 50.89 | 52.91 | |