Fig. 3

Analysis of the genetic divergence and diversity of all 78 EHV2 genes using the 20 aligned EHV2 genome sequences. A Mean % nucleotide identity. B Mean nucleotide diversity (π) values which represents the average number of nucleotide substitutions per site (excluding gaps). C Mean Ka values (representing nonsynonymous substitutions—green bars) and Ks values (representing synonymous substitutions—orange bars). Ka values < 0.002 are considered most conserved, whilst values > 0.025 are considered most diverged [77]. In panel B, the equid gammaherpesvirus (EGHV) specific genes are in red font, genes specific to both Betaherpesviruses and Gammaherpesviruses and are shaded blue, and Gammaherpesviruses specific genes are shaded yellow. Unshaded genes are conserved genes in all herpesviruses. Glycoproteins are marked with an asterisk (*). IR = internal repeats, and TR = terminal repeats