Table 1 Sequencing data quality control
From: MiRNA sequencing of Embryonic Myogenesis in Chengkou Mountain Chicken
id | Clean reads | High quality | 3’adapter null | Insert null | 5’adapter contaminants | Smaller than 18nt | PolyA | Low cutoff | Clean tags |
|---|---|---|---|---|---|---|---|---|---|
E12-1 | 15,182,867 (100%) | 15,032,507 (99.0097%) | 11,259 (0.0749%) | 27,699 (0.1843%) | 7291 (0.0485%) | 353,353 (2.3506%) | 365 (0.0024%) | 374,772 (2.4931%) | 14,257,768 (94.8462%) |
E12-2 | 15,486,862 (100%) | 15,337,952 (99.0385%) | 13,217 (0.0862%) | 25,360 (0.1653%) | 5054 (0.0330%) | 251,801 (1.6417%) | 273 (0.0018%) | 246,696 (1.6084%) | 14,795,551 (96.4637%) |
E12-3 | 13,546,502 (100%) | 13,426,336 (99.1129%) | 8751 (0.0652%) | 18,045 (0.1344%) | 4443 (0.0331%) | 203,557 (1.5161%) | 231 (0.0017%) | 230,529 (1.7170%) | 12,960,780 (96.5325%) |
E16-1 | 13,440,948 (100%) | 13,298,384 (98.9393%) | 21,028 (0.1581%) | 16,664 (0.1253%) | 4725 (0.0355%) | 200,716 (1.5093%) | 500 (0.0038%) | 215,551 (1.6209%) | 12,839,200 (96.5471%) |
E16-2 | 12,182,274 (100%) | 12,082,685 (99.1825%) | 6212 (0.0514%) | 15,911 (0.1317%) | 5505 (0.0456%) | 250,947 (2.0769%) | 302 (0.0025%) | 191,460 (1.5846%) | 11,612,348 (96.1073%) |
E16-3 | 15,876,789 (100%) | 15,709,214 (98.9445%) | 5765 (0.0367%) | 20,027 (0.1275%) | 4689 (0.0298%) | 209,133 (1.3313%) | 560 (0.0036%) | 230,806 (1.4692%) | 15,238,234 (97.0019%) |
E19-1 | 13,754,500 (100%) | 13,645,117 (99.2047%) | 5805 (0.0425%) | 19,585 (0.1435%) | 3795 (0.0278%) | 248,176 (1.8188%) | 460 (0.0034%) | 232,089 (1.7009%) | 13,135,207 (96.2631%) |
E19-2 | 15,351,792 (100%) | 15,198,195 (98.9995%) | 6913 (0.0455%) | 20,152 (0.1326%) | 4133 (0.0272%) | 247,101 (1.6259%) | 469 (0.0031%) | 211,815 (1.3937%) | 14,707,612 (96.7721%) |
E19-3 | 12,025,634 (100%) | 11,935,295 (99.2488%) | 4363 (0.0366%) | 16,022 (0.1342%) | 3712 (0.0311%) | 290,870 (2.4371%) | 264 (0.0022%) | 173,365 (1.4525%) | 11,446,699 (95.9063%) |
E21-1 | 16,230,582 (100%) | 15,989,901 (98.5171%) | 50,361 (0.3150%) | 27,090 (0.1694%) | 4495 (0.0281%) | 331,264 (2.0717%) | 504 (0.0032%) | 341,409 (2.1352%) | 15,234,778 (95.2775%) |
E21-2 | 15,518,503 (100%) | 15,363,022 (98.9981%) | 6122 (0.0398%) | 13,592 (0.0885%) | 4329 (0.0282%) | 168,750 (1.0984%) | 182 (0.0012%) | 177,280 (1.1539%) | 14,992,767 (97.5900%) |
E21-3 | 12,810,088 (100%) | 12,699,534 (99.1370%) | 3490 (0.0275%) | 19,648 (0.1547%) | 6717 (0.0529%) | 378,662 (2.9817%) | 240 (0.0019%) | 208,206 (1.6395%) | 12,082,571 (95.1418%) |