Table 2 Potential targets in the IHHNV-EVE cluster of PC7. See the supplementary information (Supplementary Figure S1) for detailed information on the BlastN results
Detection Primer | Primer sequences | Hit positions on PC7 | Target size/identity | Primer mismatch |
|---|---|---|---|---|
OIE [17] | IHHNV309F/R | Location 1: 40,036,120 - 40,036,428 nt | 309/95% | 0/22 |
Location 2: 40,037,386 - 40,037,694 nt | 309/95% | 0/22 | ||
Location 3: 40,040,184 - 40,040,492 nt | 309/95% | 0/22 | ||
Jaroenram and Owens, 2014 [18] | RPA1F | Location 1: 40,041,559 – 40,041,588 nt | 121/97% | 1/30 |
RPA1R | Location 1: 40,041,679 – 40,041,650 nt | 121/97% | 1/30 | |
Cowley et al., 2018 [19] | IHHNV-q309F1 | Location 1: 40,036,089 - 40,036,115 nt | 98/100% | 0/27 |
Location 2: 40,037,355 – 40,037,381 nt | 98/100% | 0/27 | ||
Location 3: 40,040,153 – 40,040,179 nt | 98/100% | 0/27 | ||
IHHNV-q309R1 | Location 1: 40,036,186 – 40,036,161 nt | 98/100% | 0/26 | |
Location 2: 40,037,452 – 40,037,427 nt | 98/100% | 0/26 | ||
Location 3: 40,040,250 – 40,040,225 nt | 98/100% | 0/26 | ||
IHHNV-q309Pr1 (probe) | Location 1: 40,036,119 – 40,036,136 nt | NA | 0/18 | |
Location 2: 40,037,385 – 40,037,402 nt | NA | 0/18 | ||
Location 3: 40,040,183 – 40,040,200 nt | NA | 0/18 |