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Fig. 3 | BMC Genomics

Fig. 3

From: Conceptual framework for the insect metamorphosis from larvae to pupae by transcriptomic profiling, a case study of Helicoverpa armigera (Lepidoptera: Noctuidae)

Fig. 3

Expression of genes related to the JH and ecdysone signaling pathways during metamorphosis of Helicoverpa armigera. A The regulatory model of juvenile hormone (JH) and 20E signaling pathways. B and D Heatmap of genes in the brain (B) and fat body (D) related to the JH and ecdysone signaling pathways. C and E qPCR validation of genes in the brain (C) and fat body (E) related to regulation of JH and ecdysone signaling pathways. Histograms represent the results of qPCR (left Y axis). Line charts represent the results of RNA-Seq (right Y axis). Error bars represent mean ± SD. One-way ANOVA with Tukey’s post hoc test was used for statistical analysis of qPCR results. Different lowercase letters indicated significant differences (p < 0.05). BL5D2: the brain at the feeding stage; BL5W0: the brain at the initiation of the wandering stage; BL5D5: the brain at the prepupal stage; BP0: the brain at the initiation of the pupal stage; FL5D2: the fat body at the feeding stage; FL5W0: the fat body at the initiation of the wandering stage; FL5D5: the fat body at the prepupal stage; FP0: the fat body at the initiation of the pupal stage

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