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Table 2 Genetic diversity parameters of the newly developed 33 EST-SSR markers across the 46 Monochasma savatieri individuals

From: Analysis of the genetic diversity and population structure of Monochasma savatieri Franch. ex Maxim using novel EST-SSR markers

Primer name

Primer sequence (5′-3′)

SSR motif

Na

MAF

GD

PIC

LRC-19-1

F:ATACAGTTCGCCGAGCAATC

(CT)12(CA)7

4.000

0.478

0.606

0.526

R:TGTGACAGAGCAAGCCAAAC

     

LRC-38-2

F:TATTGCGTTCTTTGCTTCCA

(T)17

8.000

0.261

0.817

0.791

R:CGACCATCACCTCTAGCTCC

     

LRC-491-1

F:AGTTAATGATGCCGATTGCC

(GA)10

5.000

0.435

0.704

0.659

R:TAATCTCCTCGCCAAATTGC

     

LRC-955-2

F:AGATGCGGTAAACCACAAGG

(T)10

5.000

0.435

0.721

0.682

R:AAGAGGGCATTGGCTTTTCT

     

LRC-1030-1

F:AAGCTCTGTAATGGCGTTCG

(CT)9

6.000

0.522

0.672

0.640

R:TGAATATGCGCTTCGATTGT

     

LRC-1279-1

F:TAACCAGAATCCACGTGTCG

(T)10

7.000

0.565

0.635

0.605

R:AAGGTCCCATAAGCATCAGC

     

LRC-3853-1

F:CTCGCACACTATCTTCCACG

(GGCA)6

7.000

0.304

0.796

0.767

R:AGGGATGATGGATACGGATG

    

LRC-4136-1

F:TCGCCATAACTCCGTCTTCT

(AG)6

6.000

0.565

0.636

0.606

R:GCGAAAGCTATGCCGACTAC

     

LRC-4412-1

F:TCCAGTTTTCCATCACCACA

(A)20

4.000

0.543

0.609

0.549

R:GCGTCGTTGTAATCATGGTG

     

LRC-6564-1

F:ATTCAGGCTTCAGCAGCAAC

(AG)16

10.000

0.130

0.894

0.884

R:GTCGTTCAGCAGTGGGATCT

     

LRC-8962-2

F:AGAACAAGTCGTCGGAGCAT

(T)21

4.000

0.489

0.619

0.547

R:TGCCTTCCCTTGACACTACA

     

LRC-9052-1

F:AGATCGATGTGCTCAGCCTT

(GC)7

4.000

0.413

0.680

0.621

R:GTCTCAACAAGCGACGTTCA

     

LRC-10353-1

F:CGATCTGCTCACAGAACCAA

(CGG)5

7.000

0.228

0.813

0.786

R:CAATCGCTGCAGATCGTTTA

     

LRC-18371-1

F:TTGACAAGGCATGCAAATTC

(TTC)5 T(TA)14

6.000

0.435

0.693

0.641

R:TGAGTTCAACCACTTGGGAG

     

LRC-18468-1

F:CCACACGCAGTATTTGGTGA

(CT)6

6.000

0.457

0.702

0.660

R:TGCTCGTAATGTGGCTCAAG

     

LRC-18556-1

F:TTGCAGAAGAATTGCATTGG

(G)10

7.000

0.435

0.712

0.671

R:CACACGCTGCAACTGGTATT

     

LRC-19013-3

F:TATTTCACTGAGGTGCGAGC

(ATGT)9

11.000

0.304

0.840

0.823

R:TAACGACCACCATTAACGCA

     

LRC-21021-1

F:TACACACAATGCATGCCTCA

(C)12

8.000

0.283

0.818

0.794

R:GCATGATCCGAAGAGGAGAA

     

LRC-22140-1

F:AGTTTGCCGACTCAGAAGGA

(G)14

7.000

0.500

0.656

0.606

R:CCTTTGCAGATTTAGAGCCG

     

LRC-24756-2

F:TCTCTGCATGCATCCACTTC

(A)11

7.000

0.391

0.758

0.726

R:GGCGACGTAGTCATGGAGTT

     

LRC-25132-1

F:AGGCGAGTTGGTTTCAGCTA

(ATT)16

8.000

0.261

0.833

0.812

R:GAAACATGCGTTTGGTGTTG

     

LRC-28075-1

F:CTTTCGCCGTTCAAGTTTTC

(T)13

6.000

0.391

0.753

0.719

R:CTCGTTCTTGAGCATGTGGA

     

LRC-34475-2

F:TTGGTGCACCTGCTATGTTT

(AT)18

7.000

0.283

0.796

0.766

R:CAGAGTGAAACCACAGCCAA

     

LRC-35320-1

F:GATTGGCCTCACTTGGTCTC

(AAATT)5

8.000

0.283

0.823

0.800

R:TTGCAATGCCATGAACAAAC

     

LRC-36979-3

F:TCCATGATTGAAGCTTCTCG

(TG)15

10.000

0.283

0.852

0.837

R:TGCAACAAGGAAGAGCAATG

     

LRC-43046-1

F:ATTCTCCGTTGGGAGGTTCT

(TA)7

8.000

0.435

0.737

0.706

R:AGGAACCAGCTGCACAATCT

     

LRC-50242-1

F:TGGAGATTACTTCGCAGCCT

(GA)11

4.000

0.457

0.651

0.584

R:CCGATGGTGATTATTGGACC

     

LRC-55216-1

F:CTGCAAGTGACATGATGGCT

(CAT)5

4.000

0.652

0.519

0.470

R:GCGGAATTGACTGTGAACCT

     

LRC-55844-1

F:ATACTCGTTCACCCAATCGC

(T)10C(CT)9

6.000

0.446

0.729

0.697

R:TAGCTCCACGATGAAAGGCT

     

LRC-60747-1

F:GAAGTGGTGGCACTGAAGGT

(TA)22

3.000

0.924

0.143

0.138

R:GCCACTGCCTCTTCTCAGAC

     

LRC-63796-1

F:CCATTTGCACTCCGGATACT

(ATTG)5

5.000

0.478

0.689

0.647

R:ATCTCCGCCACTGATGTAGG

     

LRC-68008-1

F:GGAAACCAACCTGTGCCTTA

(AT)7

6.000

0.457

0.709

0.671

R:GGTTTACGGCGAAATCTTGA

     

LRC-68284-2

F:CGGACTTCCAATTTCGCTTA

(TTTA)5

4.000

0.478

0.668

0.615

R:CCACATAGCTTTACCGGCAT

     

Mean

  

6.303

0.424

0.706

0.668

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BMC Genomics

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