Fig. 3

Analysis of all genes for which expression changed in response to acute activity. A Schematic of the experimental setup for RNA-Sequencing (RNA-seq)-based assessment of transcriptional changes caused by acute exercise using the OMC model. RNA-seq was performed on three independent biological replicates (N = 3) per condition (control and exercised). B Heatmap showing all 1190 genes that show a significant change (adjusted p-value ≤0.05) of 1.5 fold or more compared to control samples. N = 3 biological replicates per condition. The p-values are adjusted (padj) for multiple testing using Benjamini and Hochberg method. C Dot plot showing the top 25 most significantly enriched GO terms. The color represents the adjusted p-values of the enrichment analysis relative to the other displayed terms (brighter red is more significant) and the size of the terms represent the number of genes that consist the term. D An enrichment GO plot showing the relationship between the top 25 most significantly enriched GO terms based on adjusted p-value. Similar terms are grouped together. The color represents the adjusted p-values of the enrichment analysis relative to the other displayed terms (brighter red is more significant) and the size of the terms represent the number of genes that consist the term