From: Using single-worm RNA sequencing to study C. elegans responses to pathogen infection
Condition | Processing before shearing | Device of shearing |
---|---|---|
1 | No processing | TissueLyser LTb |
2 | Incubated in Lysis Buffera at 65 °C for 10 min, 85 °C for 1 min, then held at 4 °C in a thermocycler | TissueLyser LTb |
3 | Incubated in Lysis Buffera at 65 °C for 10 min, 85 °C for 1 min, then held at 4 °C in a thermocycler | QIAshredder spin columnc |