High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes

BMC Genomics

Table 3 Summary of confirmed insertion sites for each transgenic line

				Library support for insertion			Genomic context			T₂ zygosity
Line	Chromosome	Location	Transgene	Border	PCR library	Total # of reads	Gene	Description	Protein	Het:Hmz:WT
Line #1415	Chr 5	9,760,996	OGC	LB	all	7228	LOC104789082	B3 domain-containing protein At2g33720-like	Plant-specific B3-DNA binding domain	5:3:0
Line #1415	Chr 7	4,217,439	OCP1	LB	all	37	LOC104704287	mRNA-carnosine N-methyltransferase-like	Carnosine N-methyltransferase-like	8:0:0
Line #1416	Chr 8	24,442,089	OCP1	LB	1,3,4,5	279	none	non-coding region	none	19:0:0
	Chr 15	982,591	OCP1	LB & RB	1,2,4,5	2281	none	non-coding region	none	8:6:5
	Chr 18	7,461,224	OCP1	LB & RB	all	9236	LOC104763210	Ribosome biogenesis protein BOP1 homolog	Pseudogene	12:7:0
GK-269g12	Chr 1	17,683,348	NPTII	LB & RB	all	27,436	AT1G47960	Cell wall / vacuolar inhibitor of fructosidase 1	Cell-wall inhibitor of beta fructosidase and similar proteins	NA

The genomic location of the insertion and the transgene identified are presented on the left. Library support columns indicate which of the PCR libraries contributed to detection of the insertion site and the overall total number of reads obtained for each site. Genomic context columns provide information on the gene at the location of the insertion which may be altered by the introduction of T-DNA. Segregation of the transgene in the T₂ generation is reported in the last column

ISSN: 1471-2164