Table 4 Zygosity screening for T2 plants of line #1416 for the OCP1 insertion on chromosome 15
From: High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes
Chr15:982,591 | Chr18:7,461,224 | Chr8:24,442,089 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
T2 Plant | PCR A/B | Het | Hmz | WT | PCR A/B | Het | Hmz | WT | PCR A/B | Het | Hmz | WT |
1 | + / + | X | + / + | X | + / + | X | ||||||
2 | + / - | X | + / - | X | + / + | X | ||||||
3 | - / + | X | + / - | X | + / + | X | ||||||
4 | + / + | X | + / + | X | + / + | X | ||||||
5 | + / - | X | + / + | X | + / + | X | ||||||
6 | - / + | X | + / + | X | + / + | X | ||||||
7 | - / + | X | + / - | X | + / + | X | ||||||
8 | + / - | X | + / + | X | + / + | X | ||||||
9 | + / + | X | + / - | X | + / + | X | ||||||
10 | + / - | X | + / + | X | + / + | X | ||||||
11 | + / + | X | + / - | X | + / + | X | ||||||
12 | + / + | X | + / + | X | + / + | X | ||||||
13 | - / + | X | + / + | X | + / + | X | ||||||
14 | - / + | X | + / + | X | + / + | X | ||||||
15 | + / - | X | + / + | X | + / + | X | ||||||
16 | + / + | X | + / + | X | + / + | X | ||||||
17 | + / + | X | + / - | X | + / + | X | ||||||
18 | + / - | X | + / - | X | + / + | X | ||||||
19 | + / + | X | + / + | X | + / + | X | ||||||
Total trangene positive: | 14 | 19 | 19 | |||||||||
Het | Hmz | WT | Het | Hmz | WT | Het | Hmz | WT | ||||
Segregation ratio: | 8 | 6 | 5 | 12 | 7 | 0 | 19 | 0 | 0 | |||
57.14% | 42.86% | 35.71% | 63.16% | 36.84% | 0.00% | 100.00% | 0.00% | 0.00% | ||||