Fig. 10

Gene synteny of the itaconate degradation pathway operon. A Maximum likelihood tree of RipC amino acid sequence from Serratia entomophila (bold) alongside seven other gene homologues found through BlastP. Scale bar represents 20% genetic variation. Bootstrap values above 50% are shown. B ripABC synteny and gene arrangement with the depicted itaconate operons- the three gene Yersinia pestis and six gene Pseudomonas aeruginosa operons. Colours indicate genes with the same functional prediction as S. entomophila, refer Table 8 for annotations. Red arrow under ripC denotes the mutated gene. C S. proteamaculans co-location of pip and DNA polymerase III subunit theta, where in S. entomophila 626 the itaconate degradation region is positioned. GenBank protein accessions shown in brackets